Description
Extinction CoeffA280 nm = 1.0 at 1.0 mg/mLSpecificityMonospecific for C4a in human plasmaGeneral DescriptionProduct is whole polyclonal antiserum from rabbits immunized with highly purified human complement protein. Product is not a purified IgG fraction. Rabbits are maintained in FDA certified facilities.Physical Characteristics & StructureAntibodies present in the antisera are primarily IgG.ApplicationsWestern Blots: Effective at dilutions 1/500 to 1/1000 depending on conditions.Most effective against non-reduced antigen.ELISA: Effective at dilutions 1/500 to 1/2000 depending on conditions.Immunodiffusion: Effective against NHS (20 µg/ml C4a) at 1/4 dilution